The work investigated the microstructures of titanium dioxide, consisting of close-packed particles in the form of spherical particles with average sizes of 40 and 63 microns. It has been established that the spectra of single- and two-photon excited luminescence differ from each other in intensity, shape and position. It has been shown that at room temperature intense photoluminescence (2.91 eV) is observed in titanium dioxide microstructures when excited by a copper vapor laser generation line operating in a pulse-periodic mode. The developed method for recording optical spectra opens up wide possibilities for recording weak signals of secondary radiation from a wide class of dielectric and semiconductor structures necessary for solving many practical problems.